August 24, 2020
Research for Disulfide-Linked, Self-Immolating Subsequent-Technology Antibody Drug
Conjugation Site Influences Antibody-Conjugated Drug PK Assays: Case Studies for Disulfide-Linked, Self-Immolating Next-Generation Antibody Drug Conjugates
Immunoaffinity (IA) LC-MS/MS pharmacokinetic (PK) assays are widely used in the field for antibody drug conjugates (ADCs) containing peptide linkers that are enzymatically cleavable, such as MC-ValCit-PAB. Conjugate PK assay strategies for these ADCs involve cleavage with cathepsin B or papain to release and measure the antibody-conjugated drug (acDrug) concentration.
However, robust acDrug PK methods for disulfide-linked self-immolating ADCs are lacking as they are a different conjugation modality. We developed acDrug PK assays for next-generation disulfide-linked ADCs involving immunoaffinity capture, chemical cleavage, and LC-MS/MS. Disulfide-linked ADCs captured from plasma were chemically reduced at basic pH to release the linker-drug, followed by self-immolation to liberate the active drug, and quantified by MRM LC-MS/MS.
Herein, we detail the development and optimization of this chemical cleavage acDrug PK assay, resulting in robust accuracy and precision (<u>+</u>20%). The conjugation site of the linker-drug on the antibody was found to affect the kinetics of drug release.
Multiple biophysical and chemical characteristics, such as tertiary structure, fractional solvent accessibility, pKa of the conjugation site, surrounding residue’s pI, and electrostatic charge, may directly impact the drug release kinetics. Similar site-specific stability has been previously reported for ADCs in vivo.
The assay development and qualification data for this original assay format are presented along with its application to multiple in vitro and in vivo studies across species.
Gestational Dysfunction-Driven Diets and Probiotic Supplementation Correlate with the Profile of Allergen-Specific Antibodies in the Serum of Allergy Sufferers
Background: Maternal diet has significant effects on development of childhood atopic disease and hypersensitivity development. However, the gestational dysfunctions demanding special diets are becoming a widespread phenomenon, their immunological implications can be manifested in the profile of antibodies in the offspring’s serum.
Methods: 153 allergic and 150 healthy individuals were diagnosed for allergy using specific antibody and cytokine immunoassay tests. The medical history of subjects along with mothers’ course of pregnancy was completed by allergologist’s anamnesis. A self-organizing neural network and multivariate analyses to complex data and pick basic interactions were used.
Results: Two significant explanatory modules were determined. The first was formed by gestational diabetic and cholestatic diet, infant formula feeding type, probiotic supplementation and its BMI index, moderate IgE, increased IgG levels of antibodies and single or poly-food allergy type (7 clusters).
The second was formed by gestational vegan/vegetarian and elimination diet, maternal probiotic supplementation, sex, high IgE total antibodies and food and mixed poly-allergy to aero- and food-origin allergens (19 clusters).
Conclusions: Significant associations were observed between special gestational diet intake underlying foetal programming and the mechanisms of childhood allergy. The novelty is the positive association between diabetic and cholestatic diet intake and IgE/IgG-mediated food hypersensitivity.
Keywords: allergic patterns; allergy prevalence; artificial self-organizing neural network; foetal programming; gestational dysfunction-driven diets; probiotics supplementation; specific IgE-antibodies profile.
genediag
GOAT ANTI HUMAN TNF ALPHA |
|||
| MBS221500-01mg | MyBiosource | 0.1mg | EUR 660 |
GOAT ANTI HUMAN TNF ALPHA |
|||
| MBS221500-5x01mg | MyBiosource | 5x0.1mg | EUR 2795 |
MOUSE ANTI HUMAN TNF ALPHA |
|||
| MBS213142-05mg | MyBiosource | 0.5mg | EUR 1165 |
MOUSE ANTI HUMAN TNF ALPHA |
|||
| MBS213142-5x05mg | MyBiosource | 5x0.5mg | EUR 5080 |
MOUSE ANTI HUMAN TNF ALPHA |
|||
| MBS213345-02mg | MyBiosource | 0.2mg | EUR 935 |
MOUSE ANTI HUMAN TNF ALPHA |
|||
| MBS213345-5x02mg | MyBiosource | 5x0.2mg | EUR 4045 |
Tumor necrosis factor alpha TNF-? (72 - 82), human (AA: Pro-Leu-Ala-Gln-Ala-Val-Arg-Ser-Ser-Ser-Arg) (MW: 1171.33) |
|||
| SP-86635-5 | Alpha Diagnostics | 5 mg | EUR 343.2 |
Monoclonal Anti-Human Tumor Necrosis Factor-Alpha (TNF-alpha) IgG #1 aff. Pure |
|||
| TNFA11-M | Alpha Diagnostics | 100 ug | EUR 578.4 |
Monoclonal Anti-Human Tumor Necrosis Factor-Alpha (TNF-alpha) IgG #2, aff. Pure |
|||
| TNFA12-M | Alpha Diagnostics | 100 ug | EUR 578.4 |
Alpha 1-Antitrypsin, Human Plasma |
|||
| A1AT15-N | Alpha Diagnostics | 1 mg | EUR 196.8 |
Alpha 2-Antiplasmin, Human Plasma |
|||
| A2AP15-100 | Alpha Diagnostics | 100 ug | EUR 196.8 |
Recombinant (E.Coli) Human p38 alpha/SAPK2 alpha |
|||
| RP-675 | Alpha Diagnostics | 1 ug | EUR 343.2 |
Rabbit Anti-Rat TNF-alpha Converting Enzyme (TACE) antiserum |
|||
| TACE11-S | Alpha Diagnostics | 100 ul | EUR 548.4 |
Alpha 1-Antichymotrypsin , Human Plasma |
|||
| A1CT15-N-100 | Alpha Diagnostics | 100 ug | EUR 196.8 |
Rabbit Anti-Human alpha-Tubulin IgG |
|||
| TUBA13-A | Alpha Diagnostics | 100 ul | EUR 578.4 |
Goat Anti-Human Tumor Necrosis Factor-Alpha (TNF-alpha) IgG #2, aff. Pure (neutralzing) |
|||
| TNFA13-A | Alpha Diagnostics | 100 ul | EUR 578.4 |
Pig Tumor Necrosis Factor Alpha (TNF-a) ELISA Kit, 96 tests, Quantitative |
|||
| 9965 | Alpha Diagnostics | 1 Kit | EUR 927.6 |
Recombinant (E.Coli) purified human Tumor Necrosis Factor-Alpha (TNF-alpha), biologically active |
|||
| TNFA15-R-10 | Alpha Diagnostics | 10 ug | EUR 270 |
Monoclonal Anti-Human alpha-Tubulin IgG |
|||
| TUBA12-M | Alpha Diagnostics | 100 ul | EUR 578.4 |
Bovine Tumor necrosis factor alpha (TNF-A) ELISA Kit, 96 tests, Quantitative |
|||
| 9810 | Alpha Diagnostics | 1 Kit | EUR 927.6 |
Canine Tumor Necrosis Factor alpha (TNF-a) ELISA Kit, 96 tests, Quantitative |
|||
| 10460 | Alpha Diagnostics | 1 Kit | EUR 927.6 |
Rabbit Anti-human Topoisomerase II alpha (TOP2 alpha) IgG, aff pure |
|||
| TOP2A11-A | Alpha Diagnostics | 100 ug | EUR 578.4 |
Chicken Anti-Human alpha 1 anti-trypsin IgG/Y |
|||
| A1AT12-A | Alpha Diagnostics | 100 ul | EUR 578.4 |
Tumor necrosis factor alpha TNF-? (78-96) (human) (AA: His-Thr-Ile-Ser-Arg-Ile-Ala-Val-Ser-Tyr-Gln-Thr-Lys-Val-Asn-Leu-Leu-Ser-Ala) (MW: 2101.45) |
|||
| SP-89730-1 | Alpha Diagnostics | 1 mg | EUR 196.8 |
Rabbit Anti-Human p73 alpha antiserum # 2 |
|||
| P73A12-S | Alpha Diagnostics | 100 ul | EUR 548.4 |
Tumor necrosis factor alpha TNF-? (46-65) (human) (AA: Asn-Gln-Leu-Val-Val-Pro-Ser-Glu-Gly-Leu-Tyr-Leu-Ile-Tyr-Ser-Gln-Val-Leu-Phe-Lys) (MW: 2310.74) |
|||
| SP-89728-1 | Alpha Diagnostics | 1 mg | EUR 489.6 |
Rabbit Anti-Human Glutathione Transferase alpha (GST-alpha) antiserum |
|||
| GSTA12-S | Alpha Diagnostics | 100 ul | EUR 548.4 |
Guinea pig Tumor Necrosis Factor Alpha (TNF-a) ELISA Kit, 96 tests, Quantitative |
|||
| 9870 | Alpha Diagnostics | 1 Kit | EUR 927.6 |
Tumor necrosis factor alpha TNF-? (31 - 45), human (AA: Arg-Arg-Ala-Asn-Ala-Leu-Leu-Ala-Asn-Gly-Val-Glu-Leu-Arg-Asp) (MW: 1667.90) |
|||
| SP-86636-1 | Alpha Diagnostics | 1 mg | EUR 196.8 |
Human Alpha-Thrombin (human plasma), purified |
|||
| TBNA16-N-100 | Alpha Diagnostics | 100 ug | EUR 196.8 |
human Topoisomerase II alpha (TOP2 alpha) Control/blocking peptide |
|||
| TOP2A11-P | Alpha Diagnostics | 100 ug | EUR 196.8 |
Recombinant Human Alpha-Synuclein |
|||
| RP-847 | Alpha Diagnostics | 20 ug | EUR 196.8 |
Rabbit Anti-Human Estrogen receptor alpha (ER-alpha) peptide IgG |
|||
| ERA12-A | Alpha Diagnostics | 100 ug | EUR 578.4 |
Recombinant purified human TNF Receptor 2 (TNFR2/TNF-RII, Etannercept/TNF-R75, p75TNFR) protein (E.Coli, His-tag) |
|||
| TNFR25-R-20 | Alpha Diagnostics | 10 ug | EUR 343.2 |
Rabbit Anti-Human p73 alpha/beta antiserum # 1 |
|||
| P73A11-S | Alpha Diagnostics | 100 ul | EUR 548.4 |
Recombinant purified, human Interleukin-1 alpha (IL-1 alpha), active |
|||
| IL1A15-R-10 | Alpha Diagnostics | 10 ug | EUR 416.4 |
Monoclonal anti-human Alpha (FSH-Alpha), IgG clone 3 aff pure |
|||
| FSHA12-M | Alpha Diagnostics | 1 mg | EUR 578.4 |
Goat Anti-human Alpha fetoprotein (AFP) IgG, pure |
|||
| AFP13-A | Alpha Diagnostics | 1 mg | EUR 781.2 |
Goat Anti-Human Lutenizing Hormone Alpha (LH-Alpha) IgG aff pure |
|||
| LHA13-A | Alpha Diagnostics | 1 mg | EUR 343.2 |
Tumor necrosis factor alpha TNF-? (10-36) (human) (AA: Asp-Lys-Pro-Val-Ala-His-Val-Val-Ala-Asn-Pro-Gln-Ala-Glu-Gly-Gln-Leu-Gln-Trp-Leu-Asn-Arg-Arg-Ala-Asn-Ala-Leu) (MW: 2996.41) |
|||
| SP-89727-1 | Alpha Diagnostics | 1 mg | EUR 270 |
Rabbit Anti-Rat TNF-alpha Converting Enzyme (TACE), IgG aff. Pure |
|||
| TACE11-A | Alpha Diagnostics | 100 ug | EUR 578.4 |
Goat Anti-human Chorionic Gonadotropin Alpha (hCG-Alpha) IgG aff pure |
|||
| HCGA13-A | Alpha Diagnostics | 1 mg | EUR 343.2 |
Rabbit Anti-Human HE2 alpha IgG #1, aff pure |
|||
| HE2A11-A | Alpha Diagnostics | 100 ug | EUR 578.4 |
Rabbit Anti-Human p73 alpha/ IgG # 2, aff pure |
|||
| P73A12-A | Alpha Diagnostics | 100 ug | EUR 578.4 |
Rabbit Anti-Human alpha-Tubulin IgG-HRP Conjugate |
|||
| TUBA13-HRP | Alpha Diagnostics | 100 ul | EUR 518.4 |
Recombinant (E.Coli) Human Thyrostimulin Alpha |
|||
| RP-595 | Alpha Diagnostics | 10 ug | EUR 343.2 |
Rabbit Anti-Human alpha-Tubulin IgG-Biotin Conjugate |
|||
| TUBA13-BTN | Alpha Diagnostics | 100 ul | EUR 562.8 |
Alpha 1-Antitrypsin (A1AT), Human protein control for WB |
|||
| A1AT11-C | Alpha Diagnostics | 100 ul | EUR 343.2 |
Alpha 2-HS Glycoprotein, Human Plasma |
|||
| A2HG15-N | Alpha Diagnostics | 1 mg | EUR 343.2 |
Alpha 2-Macroglobulin (A2M), Human Plasma |
|||
| A2MG15-N | Alpha Diagnostics | 1 mg | EUR 196.8 |
Goat Anti-Human Alpha 1-Antitrypsin (A1AT) IgG, unlabeled |
|||
| A1AT11-A | Alpha Diagnostics | 100 ul | EUR 489.6 |
Rabbit Anti-Human Resistin-Like protein alpha (RELM-alpha/FIZZ1) antiserum #2 |
|||
| RELMA12-S | Alpha Diagnostics | 100 ul | EUR 548.4 |
Chicken Anti-Human Alpha 2 Macroglobulin (A2M) IgG/Y |
|||
| A2MG13-A | Alpha Diagnostics | 100 ul | EUR 578.4 |
Recombinant (E.Coli) Human Crystallin Alpha A |
|||
| RP-611 | Alpha Diagnostics | 20 ug | EUR 196.8 |
Recombinant (E.Coli) Human Crystallin Alpha B |
|||
| RP-612 | Alpha Diagnostics | 20 ug | EUR 196.8 |
Human Alpha Feto Protein (AFP) High Pure |
|||
| AFP15-N | Alpha Diagnostics | 1 mg | EUR 854.4 |
Monoclonal Anti-Human Chorionic Gonadotropin Alpha (hCG-alpha) IgG #1, aff pure |
|||
| HCGA11-M | Alpha Diagnostics | 1 mg | EUR 343.2 |
HUMAN ANTI TNF ALPHA (INFLIXIMAB BIOSIMILAR) |
|||
| MBS226034-02mg | MyBiosource | 0.2mg | EUR 715 |
HUMAN ANTI TNF ALPHA (INFLIXIMAB BIOSIMILAR) |
|||
| MBS226034-5x02mg | MyBiosource | 5x0.2mg | EUR 3045 |
Rabbit Anti-Human p73 alpha/beta IgG # 1, aff pure |
|||
| P73A11-A | Alpha Diagnostics | 100 ug | EUR 578.4 |
Anti-Human TNF alpha |
|||
| GWB-619378 | GenWay Biotech | 1 mg | Ask for price |
Anti-Human TNF alpha |
|||
| GWB-8F48ED | GenWay Biotech | 1 mL | Ask for price |
Anti-Human TNF-&alpha |
|||
| GWB-BIG93C | GenWay Biotech | 500ug | Ask for price |
Monoclonal Anti-human Chronionic Gonadotropin Alpha (hCG-Alpha) IgG #2, caff pure |
|||
| HCGA12-M | Alpha Diagnostics | 1 mg | EUR 343.2 |
Mouse Monoclonal Anti-human Alpha fetoprotein (AFP) (clone 1) |
|||
| AFP11-M | Alpha Diagnostics | 1 mg | EUR 578.4 |
Mouse Monoclonal Anti-human Alpha fetoprotein (AFP) (clone 2) |
|||
| AFP12-M | Alpha Diagnostics | 1 mg | EUR 578.4 |
Human Alpha Synuclein (A30P) Recombinant protein |
|||
| SYN-A30P-R | Alpha Diagnostics | 50ug | EUR 315.6 |
Human Alpha Synuclein (A53T) Recombinant protein |
|||
| SYN-A53T-R | Alpha Diagnostics | 50ug | EUR 315.6 |
Recombinant (E.Coli) Human Estrogen Receptor Alpha |
|||
| RP-940 | Alpha Diagnostics | 2 ug | EUR 416.4 |
Rabbit Anti-Human Alpha Defensin 4 (NP-4) antiserum #1 |
|||
| HDEFA41-S | Alpha Diagnostics | 100 ul | EUR 548.4 |
Rabbit Anti-Human Alpha Defensin 5 (NP-5),antiserum #1 |
|||
| HDEFA51-S | Alpha Diagnostics | 100 ul | EUR 548.4 |
Rabbit Anti-Human Alpha Defensin 6 (NP-6) antiserum #1 |
|||
| HDEFA61-S | Alpha Diagnostics | 100 ul | EUR 548.4 |
Recombinant (E.Coli) purified human Tumor Necrosis Factor-Alpha Variant (TNF-alpha variant, 151-aa), biologically active |
|||
| TNFA16-R-10 | Alpha Diagnostics | 10 ug | EUR 270 |
Human HE2 alpha Control/blocking peptide #1 |
|||
| HE2A11-P | Alpha Diagnostics | 100 ug | EUR 196.8 |
Human p73 alpha Control/blocking peptide #2 |
|||
| P73A12-P | Alpha Diagnostics | 100 ug | EUR 196.8 |
Sheep Anti-Human alpha 1 antichymotrypsin (ACT) IgG, aff pure |
|||
| ACT11-A | Alpha Diagnostics | 100 ug | EUR 489.6 |
Rabbit Anti-Human GABAa Receptor alpha 1 (GAA1) Antiserum #1 |
|||
| GAA11-S | Alpha Diagnostics | 100 ul | EUR 548.4 |
Monoclonal anti-human Lutenizing Hormone Alpha (LH-Alpha) IgG, clone 2 aff pure |
|||
| LHA12-M | Alpha Diagnostics | 1 mg | EUR 343.2 |
Recombinant (E.Coli) Human Casein Kinase 2 alpha |
|||
| RP-666 | Alpha Diagnostics | 3 ug | EUR 708 |
Recombinant (Sf9) Human Protein Kinase C alpha |
|||
| RP-676 | Alpha Diagnostics | 10 ug | EUR 343.2 |
Goat Anti-Human Alpha 1-Antitrypsin (A1AT) IgG, HRP conjugate |
|||
| A1AT11-HRP | Alpha Diagnostics | 100 ul | EUR 489.6 |
Goat Anti-Human IgA (alpha chain sp.) IgG-Biotin Conjugate |
|||
| 10040 | Alpha Diagnostics | 0.5 mg | EUR 242.4 |
Goat Anti-Ferret IgA (alpha), unlabeled |
|||
| 70519 | Alpha Diagnostics | 0.5 mg | EUR 255.6 |
Filling the Antibody Pipeline in Allergy: PIPE Cloning of IgE, IgG 1 and IgG 4 against the Major Birch Pollen Allergen Bet v 1
Birch pollen allergy is among the most prevalent pollen allergies in Northern and Central Europe. This IgE-mediated disease can be treated with allergen immunotherapy (AIT), which typically gives rise to IgG antibodies inducing tolerance. Although the main mechanisms of allergen immunotherapy (AIT) are known, questions regarding possible Fc-mediated effects of IgG antibodies remain unanswered.
This can mainly be attributed to the unavailability of appropriate tools, i.e., well-characterised recombinant antibodies (rAbs). We hereby aimed at providing human rAbs of several classes for mechanistic studies and as possible candidates for passive immunotherapy.
We engineered IgE, IgG1, and IgG4 sharing the same variable region against the major birch pollen allergen Bet v 1 using Polymerase Incomplete Primer Extension (PIPE) cloning. We tested IgE functionality and IgG blocking capabilities using appropriate model cell lines.
In vitro studies showed IgE engagement with FcεRI and CD23 and Bet v 1-dependent degranulation. Overall, we hereby present fully functional, human IgE, IgG1, and IgG4 sharing the same variable region against Bet v 1 and showcase possible applications in first mechanistic studies.
Furthermore, our IgG antibodies might be useful candidates for passive immunotherapy of birch pollen allergy.
Recombinant Human LEC |
|||
| MBS7608806-1mg | MyBiosource | 1mg | EUR 1800 |
Recombinant Human LEC |
|||
| MBS7608806-5x1mg | MyBiosource | 5x1mg | EUR 6955 |
Human LEC (CCL16) Recombinant |
|||
| MBS553045-0005mg | MyBiosource | 0.005mg | EUR 165 |
Human LEC (CCL16) Recombinant |
|||
| MBS553045-0025mg | MyBiosource | 0.025mg | EUR 245 |
Recombinant Human LEC (CCL16) |
|||
| MBS4159662-0025mg | MyBiosource | 0.025mg | EUR 385 |
Recombinant Human LEC (CCL16) |
|||
| MBS4159662-1mg | MyBiosource | 1(mg) | EUR 4600 |
Recombinant Human LEC (CCL16) |
|||
| MBS4159662-5x1mg | MyBiosource | 5x1mg | EUR 20445 |
Recombinant Human LEC (CCL16) |
|||
| Z100325 | ABM | 25 µg | EUR 235 |
|
Description: CCL16 is a CC chemokine that specifically attracts lymphocytes, dendritic cells, and monocytes; increases their adhesive properties and has myelosuppressive activity. It is constitutively expressed in liver and is increased by interleukin 10 (IL-10) in activated monocytes. CCL16 is present in human plasma suggesting that it may be active outside hepatic tissue. CCR1, CCR2, CCR5, and CCR8 are the functional receptors of this chemokine. |
|||
Recombinant Human LEC (CCL16) |
|||
| Z100329 | ABM | 1.0 mg | EUR 3900 |
|
Description: CCL16 is a CC chemokine that specifically attracts lymphocytes, dendritic cells, and monocytes; increases their adhesive properties and has myelosuppressive activity. It is constitutively expressed in liver and is increased by interleukin 10 (IL-10) in activated monocytes. CCL16 is present in human plasma suggesting that it may be active outside hepatic tissue. CCR1, CCR2, CCR5, and CCR8 are the functional receptors of this chemokine. |
|||
Human LEC/CCL16 Antibody Pair Set |
|||
| ABPR-ZB293 | Creative Diagnostics | 5 plates, 15 plates | Ask for price |
|
Description: Quantitative determination of Human LEC/CCL16 |
|||
Anti-Human LEC/NCC-4 Antibody |
|||
| 102-P51G | ReliaTech | 100 µg | EUR 245.7 |
|
Description: LEC or NCC-4 is a CC chemokine that can signal through the CCR8 and CCR1 receptors. It is expressed in the liver, spleen, and thymus. LEC is chemotactic towards monocytes and lymphocytes but not neutrophils. Recombinant human LEC is an 11.2 kDa protein containing 97 amino acid residues, including the four conserved cysteine residues present in CC chemokines. |
|||
Anti-Human LEC Goat Polyclonal Antibody |
|||
| TA328365 | Origene Technologies GmbH | 100 µg | Ask for price |
Human LEC/NCC-4 (CCL16) |
|||
| MBS692056-0005mg | MyBiosource | 0.005mg | EUR 300 |
Human LEC/NCC-4 (CCL16) |
|||
| MBS692056-002mg | MyBiosource | 0.02mg | EUR 450 |
Human LEC/NCC-4 (CCL16) |
|||
| MBS692056-5x002mg | MyBiosource | 5x0.02mg | EUR 1725 |
Recombinant Human LEC/CCL16 protein |
|||
| CD01396-5ug | Jiaxing Korain Biotech Ltd (BT Labs) | 5ug | EUR 176 |
|
Description: Human CCL16, also called Liver-expressed chemokine (LEC), Monotactin-1 (MTN-1), IL-10-inducible chemokine and so on, is expressed by the CCL16 gene located on the chromosome 17 in humans. The gene encodes a 120 a.a. residue precursor protein with a 23 a.a. residue predicted signal peptide that is cleaved to generate a 97 a.a. residue mature protein. The protein is secreted by the liver, thymus, spleen cells and showing chemotactic activity for lymphocytes and monocytes but it is distantly related to other CC chemokines, exhibiting less than 30 % sequence identity. CCL16 is highly induced by IL-10, IFN-γ and bacterial lipopolysaccharide in monmcytes and signal through CCR1, CCR2, CCR5, and CCR8. |
|||
Recombinant Human LEC (CCL16) Protein |
|||
| PROTO15467-1 | BosterBio | 20ug | EUR 380.4 |
|
Description: LEC is a CC chemokine that can signal through the CCR8 and CCR1 receptors. It is expressed in the liver, spleen, and thymus. LEC is chemotactic towards monocytes and lymphocytes but not neutrophils. Recombinant human LEC is an 11.2 kDa protein containing 97 amino acid residues, including the four conserved cysteine residues present in CC chemokines. |
|||
LEC/NCC-4 Human Recombinant |
|||
| rAP-0241 | Angio Proteomie | Inquiry | Ask for price |
Human LEC Recombinant Protein Lyophilized |
|||
| IHULECRLY5UG | Innovative research | each | EUR 341 |
|
Description: Human LEC Recombinant Protein Lyophilized |
|||
Human LEC Recombinant Protein Lyophilized |
|||
| MBS8432637-INQUIRE | MyBiosource | INQUIRE | Ask for price |
LEC antibody |
|||
| 70R-LG003 | Fitzgerald | 50 ug | EUR 312 |
|
Description: Affinity purified Goat polyclonal LEC antibody |
|||
LEC antibody |
|||
| 10R-L115A | Fitzgerald | 500 ug | EUR 240 |
|
Description: Mouse monoclonal LEC antibody |
|||
LEC Antibody |
|||
| CSB-PA916043XA01DOA-02mg | Cusabio | 0.2mg | Ask for price |
|
Description: Recombinant Arabidopsis thaliana LEC protein |
|||
LEC Antibody |
|||
| CSB-PA916043XA01DOA-10mg | Cusabio | 10mg | Ask for price |
|
Description: Recombinant Arabidopsis thaliana LEC protein |
|||
LEC antibody |
|||
| MBS532318-05mg | MyBiosource | 0.5mg | EUR 370 |
LEC antibody |
|||
| MBS532318-5x05mg | MyBiosource | 5x0.5mg | EUR 1525 |
LEC antibody |
|||
| MBS535708-005mg | MyBiosource | 0.05mg | EUR 465 |
LEC antibody |
|||
| MBS535708-5x005mg | MyBiosource | 5x0.05mg | EUR 1945 |
Biotinylated Anti-Human LEC Goat Polyclonal Antibody |
|||
| TA328364 | Origene Technologies GmbH | 50 µg | Ask for price |
Recombinant Human LEC/NCC-4 (CCL16) |
|||
| 7-01792 | CHI Scientific | 5µg | Ask for price |
Recombinant Human LEC/NCC-4 (CCL16) |
|||
| 7-01793 | CHI Scientific | 20µg | Ask for price |
Recombinant Human LEC/NCC-4 (CCL16) |
|||
| 7-01794 | CHI Scientific | 1mg | Ask for price |
Recombinant Human LEC/NCC-4 (CCL16) |
|||
| HECCP-1601 | Cyagen | 5ug | Ask for price |
Recombinant Human LEC/NCC-4 (CCL16) |
|||
| MBS143272-0005mg | MyBiosource | 0.005mg | EUR 240 |
Recombinant Human LEC/NCC-4 (CCL16) |
|||
| MBS143272-002mg | MyBiosource | 0.02mg | EUR 310 |
Recombinant Human LEC/NCC-4 (CCL16) |
|||
| MBS143272-1mg | MyBiosource | 1mg | EUR 2880 |
Recombinant Human LEC/NCC-4 (CCL16) |
|||
| MBS143272-5x1mg | MyBiosource | 5x1mg | EUR 12630 |
LEC / CCL16 / HCC4 Monoclonal antibody [Clone: LEC-14] |
|||
| LEC-14-100ug | Biologicals | 100 ug | EUR 141.79 |
|
Description: Identify and measure human LEC. Marker for inflammatory processes. |
|||
LEC / CCL16 / HCC4 Monoclonal antibody [Clone: LEC-14] |
|||
| LEC-14-1mg | Biologicals | 1 mg | EUR 897.6 |
|
Description: Identify and measure human LEC. Marker for inflammatory processes. |
|||
LEC / CCL16 / HCC4 Monoclonal antibody [Clone: LEC-14] |
|||
| LEC-14-500ug | Biologicals | 500 ug | EUR 479.6 |
|
Description: Identify and measure human LEC. Marker for inflammatory processes. |
|||
LEC / CCL16 / HCC4 Monoclonal antibody [Clone: LEC-57] |
|||
| LEC-57-100ug | Biologicals | 100 ug | EUR 141.79 |
|
Description: Identify and measure human LEC. Marker for inflammatory processes. |
|||
LEC / CCL16 / HCC4 Monoclonal antibody [Clone: LEC-57] |
|||
| LEC-57-1mg | Biologicals | 1 mg | EUR 897.6 |
|
Description: Identify and measure human LEC. Marker for inflammatory processes. |
|||
LEC / CCL16 / HCC4 Monoclonal antibody [Clone: LEC-57] |
|||
| LEC-57-500ug | Biologicals | 500 ug | EUR 479.6 |
|
Description: Identify and measure human LEC. Marker for inflammatory processes. |
|||
lec-7 Antibody |
|||
| CSB-PA600640XA01CXY-02mg | Cusabio | 0.2mg | Ask for price |
|
Description: Recombinant Caenorhabditis elegans lec-7 protein |
|||
lec-7 Antibody |
|||
| CSB-PA600640XA01CXY-10mg | Cusabio | 10mg | Ask for price |
|
Description: Recombinant Caenorhabditis elegans lec-7 protein |
|||
lec-3 Antibody |
|||
| CSB-PA601014XA01CXY-02mg | Cusabio | 0.2mg | Ask for price |
|
Description: Recombinant Caenorhabditis elegans lec-3 protein |
|||
lec-3 Antibody |
|||
| CSB-PA601014XA01CXY-10mg | Cusabio | 10mg | Ask for price |
|
Description: Recombinant Caenorhabditis elegans lec-3 protein |
|||
lec-8 Antibody |
|||
| CSB-PA222370XA01CXY-02mg | Cusabio | 0.2mg | Ask for price |
|
Description: Recombinant Caenorhabditis elegans lec-8 protein |
|||
lec-8 Antibody |
|||
| CSB-PA222370XA01CXY-10mg | Cusabio | 10mg | Ask for price |
|
Description: Recombinant Caenorhabditis elegans lec-8 protein |
|||
lec-1 Antibody |
|||
| CSB-PA330605XA01CXY-02mg | Cusabio | 0.2mg | Ask for price |
|
Description: Recombinant Caenorhabditis elegans lec-1 protein |
|||
lec-1 Antibody |
|||
| CSB-PA330605XA01CXY-10mg | Cusabio | 10mg | Ask for price |
|
Description: Recombinant Caenorhabditis elegans lec-1 protein |
|||
LEC/CCL16 Matched ELISA Antibody Pair Set, Human |
|||
| MBS8118261-15Plates | MyBiosource | 15Plates | EUR 600 |
LEC/CCL16 Matched ELISA Antibody Pair Set, Human |
|||
| MBS8118261-5Plates | MyBiosource | 5Plates | EUR 345 |
LEC/CCL16 Matched ELISA Antibody Pair Set, Human |
|||
| MBS8118261-5x15Plates | MyBiosource | 5x15Plates | EUR 2755 |
HCC-4, LEC, NCC-4, CCL16, human |
|||
| RC315-27 | Bio Basic | 5ug | EUR 125.26 |
Human LEC/NCC-4 (CCL16) Recombinant Protein |
|||
| 100-060 | ReliaTech | 20 µg | EUR 196.35 |
|
Description: LEC is a CC chemokine that can signal through the CCR8 and CCR1 receptors. It is expressed in the liver, spleen, and thymus. LEC is chemotactic towards monocytes and lymphocytes but not neutrophils. Recombinant human LEC is an 11.2 kDa protein containing 97 amino acid residues, including the four conserved cysteine residues present in CC chemokines. |
|||
Human LEC/NCC-4 (CCL16) Recombinant Protein |
|||
| 100-060S | ReliaTech | 5 µg | EUR 92.4 |
|
Description: LEC is a CC chemokine that can signal through the CCR8 and CCR1 receptors. It is expressed in the liver, spleen, and thymus. LEC is chemotactic towards monocytes and lymphocytes but not neutrophils. Recombinant human LEC is an 11.2 kDa protein containing 97 amino acid residues, including the four conserved cysteine residues present in CC chemokines. |
|||
LEC (CCL16) (NM_004590) Human Over-expression Lysate |
|||
| E45H18931-2 | EnoGene | 20 ug | EUR 395 |
LEC (CCL16) (NM_004590) Human Over-expression Lysate |
|||
| LS006360 | BosterBio | 100ug | EUR 628 |
|
Description: Transient overexpression lysate of chemokine (C-C motif) ligand 16 (CCL16) |
|||
LEC/CCL16 Protein, Human, Recombinant (His Tag) |
|||
| E45H52205E1-50 | EnoGene | 50 ug | EUR 670.31 |
LEC/CCL16 Protein, Human, Recombinant (His Tag) |
|||
| MBS8120314-005mg | MyBiosource | 0.05mg | EUR 340 |
LEC/CCL16 Protein, Human, Recombinant (His Tag) |
|||
| MBS8120314-1mg | MyBiosource | 1mg | EUR 2530 |
LEC/CCL16 Protein, Human, Recombinant (His Tag) |
|||
| MBS8120314-5x1mg | MyBiosource | 5x1mg | EUR 11240 |
LEC/CCL16 Protein, Human, Recombinant (His Tag) |
|||
| MBS8120315-005mg | MyBiosource | 0.05mg | EUR 340 |
LEC/CCL16 Protein, Human, Recombinant (His Tag) |
|||
| MBS8120315-5x005mg | MyBiosource | 5x0.05mg | EUR 1380 |
CCL16 LEC/NCC-4 Human Recombinant Protein |
|||
| PROTO15467 | BosterBio | Regular: 20ug | EUR 250 |
|
Description: CCL16 Human Recombinant produced in E. coli is a non-glycosylated, Polypeptide chain containing 97 amino acids and having a molecular mass of 11.2 kDa. The CCL16 is purified by proprietary chromatographic techniques. |
|||
LEC, NCC-4, Human (Liver-Expressed Chemokine) |
|||
| MBS635010-0005mg | MyBiosource | 0.005mg | EUR 340 |
LEC, NCC-4, Human (Liver-Expressed Chemokine) |
|||
| MBS635010-002mg | MyBiosource | 0.02mg | EUR 490 |
LEC, NCC-4, Human (Liver-Expressed Chemokine) |
|||
| MBS635010-5x002mg | MyBiosource | 5x0.02mg | EUR 2055 |
LEC protein |
|||
| 30R-AL013 | Fitzgerald | 20 ug | EUR 312 |
|
Description: Purified recombinant Human LEC protein |
|||
LEC protein |
|||
| MBS539381-002mg | MyBiosource | 0.02mg | EUR 465 |
LEC protein |
|||
| MBS539381-5x002mg | MyBiosource | 5x0.02mg | EUR 1945 |
