August 24, 2020
Research for Disulfide-Linked, Self-Immolating Subsequent-Technology Antibody Drug
Conjugation Site Influences Antibody-Conjugated Drug PK Assays: Case Studies for Disulfide-Linked, Self-Immolating Next-Generation Antibody Drug Conjugates
Immunoaffinity (IA) LC-MS/MS pharmacokinetic (PK) assays are widely used in the field for antibody drug conjugates (ADCs) containing peptide linkers that are enzymatically cleavable, such as MC-ValCit-PAB. Conjugate PK assay strategies for these ADCs involve cleavage with cathepsin B or papain to release and measure the antibody-conjugated drug (acDrug) concentration.
However, robust acDrug PK methods for disulfide-linked self-immolating ADCs are lacking as they are a different conjugation modality. We developed acDrug PK assays for next-generation disulfide-linked ADCs involving immunoaffinity capture, chemical cleavage, and LC-MS/MS. Disulfide-linked ADCs captured from plasma were chemically reduced at basic pH to release the linker-drug, followed by self-immolation to liberate the active drug, and quantified by MRM LC-MS/MS.
Herein, we detail the development and optimization of this chemical cleavage acDrug PK assay, resulting in robust accuracy and precision (<u>+</u>20%). The conjugation site of the linker-drug on the antibody was found to affect the kinetics of drug release.
Multiple biophysical and chemical characteristics, such as tertiary structure, fractional solvent accessibility, pKa of the conjugation site, surrounding residue’s pI, and electrostatic charge, may directly impact the drug release kinetics. Similar site-specific stability has been previously reported for ADCs in vivo.
The assay development and qualification data for this original assay format are presented along with its application to multiple in vitro and in vivo studies across species.
Gestational Dysfunction-Driven Diets and Probiotic Supplementation Correlate with the Profile of Allergen-Specific Antibodies in the Serum of Allergy Sufferers
Background: Maternal diet has significant effects on development of childhood atopic disease and hypersensitivity development. However, the gestational dysfunctions demanding special diets are becoming a widespread phenomenon, their immunological implications can be manifested in the profile of antibodies in the offspring’s serum.
Methods: 153 allergic and 150 healthy individuals were diagnosed for allergy using specific antibody and cytokine immunoassay tests. The medical history of subjects along with mothers’ course of pregnancy was completed by allergologist’s anamnesis. A self-organizing neural network and multivariate analyses to complex data and pick basic interactions were used.
Results: Two significant explanatory modules were determined. The first was formed by gestational diabetic and cholestatic diet, infant formula feeding type, probiotic supplementation and its BMI index, moderate IgE, increased IgG levels of antibodies and single or poly-food allergy type (7 clusters).
The second was formed by gestational vegan/vegetarian and elimination diet, maternal probiotic supplementation, sex, high IgE total antibodies and food and mixed poly-allergy to aero- and food-origin allergens (19 clusters).
Conclusions: Significant associations were observed between special gestational diet intake underlying foetal programming and the mechanisms of childhood allergy. The novelty is the positive association between diabetic and cholestatic diet intake and IgE/IgG-mediated food hypersensitivity.
Keywords: allergic patterns; allergy prevalence; artificial self-organizing neural network; foetal programming; gestational dysfunction-driven diets; probiotics supplementation; specific IgE-antibodies profile.
genediag
Human TNF-alpha ELISA Kit, 96 tests, Quantitative |
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| 100-215-TNH | Alpha Diagnostics | 1 kit | EUR 578.4 |
Pig Tumor Necrosis Factor Alpha (TNF-a) ELISA Kit, 96 tests, Quantitative |
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| 9965 | Alpha Diagnostics | 1 Kit | EUR 927.6 |
Human anti TNF Alpha autoantibody ELISA kit |
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| E01A2132-192T | BlueGene | 192 tests | EUR 1524 |
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Description: A sandwich ELISA for quantitative measurement of Human anti TNF Alpha autoantibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Human anti TNF Alpha autoantibody ELISA kit |
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| E01A2132-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
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Description: A sandwich ELISA for quantitative measurement of Human anti TNF Alpha autoantibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Human anti TNF Alpha autoantibody ELISA kit |
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| E01A2132-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
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Description: A sandwich ELISA for quantitative measurement of Human anti TNF Alpha autoantibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Rabbit Anti-human Topoisomerase II alpha (TOP2 alpha) IgG, aff pure |
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| TOP2A11-A | Alpha Diagnostics | 100 ug | EUR 578.4 |
Canine Tumor Necrosis Factor alpha (TNF-a) ELISA Kit, 96 tests, Quantitative |
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| 10460 | Alpha Diagnostics | 1 Kit | EUR 927.6 |
Bovine Tumor necrosis factor alpha (TNF-A) ELISA Kit, 96 tests, Quantitative |
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| 9810 | Alpha Diagnostics | 1 Kit | EUR 927.6 |
Antibody for Human TNF alpha |
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| SPC-1315D | Stressmarq | 0.1ml | EUR 376.8 |
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Description: A polyclonal antibody for TNF alpha from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with Human Synthesized peptide derived from internal of human TNFA.. The Antibody is tested and validated for IHC, ICC/IF, ELISA assays with the following recommended dilutions: IHC (1:100), ICC/IF (1:500), ELISA (1:10000). This TNF alpha antibody is unconjugated. |
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Antibody for Human TNF alpha |
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| SPC-1315D-A390 | Stressmarq | 0.1ml | EUR 433.2 |
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Description: A polyclonal antibody for TNF alpha from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with Human Synthesized peptide derived from internal of human TNFA.. The Antibody is tested and validated for IHC, ICC/IF, ELISA assays with the following recommended dilutions: IHC (1:100), ICC/IF (1:500), ELISA (1:10000). This TNF alpha antibody is conjugated to ATTO 390. |
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Antibody for Human TNF alpha |
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| SPC-1315D-A488 | Stressmarq | 0.1ml | EUR 432 |
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Description: A polyclonal antibody for TNF alpha from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with Human Synthesized peptide derived from internal of human TNFA.. The Antibody is tested and validated for IHC, ICC/IF, ELISA assays with the following recommended dilutions: IHC (1:100), ICC/IF (1:500), ELISA (1:10000). This TNF alpha antibody is conjugated to ATTO 488. |
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Antibody for Human TNF alpha |
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| SPC-1315D-A565 | Stressmarq | 0.1ml | EUR 432 |
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Description: A polyclonal antibody for TNF alpha from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with Human Synthesized peptide derived from internal of human TNFA.. The Antibody is tested and validated for IHC, ICC/IF, ELISA assays with the following recommended dilutions: IHC (1:100), ICC/IF (1:500), ELISA (1:10000). This TNF alpha antibody is conjugated to ATTO 565. |
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Antibody for Human TNF alpha |
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| SPC-1315D-A594 | Stressmarq | 0.1ml | EUR 432 |
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Description: A polyclonal antibody for TNF alpha from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with Human Synthesized peptide derived from internal of human TNFA.. The Antibody is tested and validated for IHC, ICC/IF, ELISA assays with the following recommended dilutions: IHC (1:100), ICC/IF (1:500), ELISA (1:10000). This TNF alpha antibody is conjugated to ATTO 594. |
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Antibody for Human TNF alpha |
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| SPC-1315D-A633 | Stressmarq | 0.1ml | EUR 432 |
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Description: A polyclonal antibody for TNF alpha from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with Human Synthesized peptide derived from internal of human TNFA.. The Antibody is tested and validated for IHC, ICC/IF, ELISA assays with the following recommended dilutions: IHC (1:100), ICC/IF (1:500), ELISA (1:10000). This TNF alpha antibody is conjugated to ATTO 633. |
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Antibody for Human TNF alpha |
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| SPC-1315D-A655 | Stressmarq | 0.1ml | EUR 432 |
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Description: A polyclonal antibody for TNF alpha from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with Human Synthesized peptide derived from internal of human TNFA.. The Antibody is tested and validated for IHC, ICC/IF, ELISA assays with the following recommended dilutions: IHC (1:100), ICC/IF (1:500), ELISA (1:10000). This TNF alpha antibody is conjugated to ATTO 655. |
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Antibody for Human TNF alpha |
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| SPC-1315D-A680 | Stressmarq | 0.1ml | EUR 432 |
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Description: A polyclonal antibody for TNF alpha from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with Human Synthesized peptide derived from internal of human TNFA.. The Antibody is tested and validated for IHC, ICC/IF, ELISA assays with the following recommended dilutions: IHC (1:100), ICC/IF (1:500), ELISA (1:10000). This TNF alpha antibody is conjugated to ATTO 680. |
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Antibody for Human TNF alpha |
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| SPC-1315D-A700 | Stressmarq | 0.1ml | EUR 432 |
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Description: A polyclonal antibody for TNF alpha from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with Human Synthesized peptide derived from internal of human TNFA.. The Antibody is tested and validated for IHC, ICC/IF, ELISA assays with the following recommended dilutions: IHC (1:100), ICC/IF (1:500), ELISA (1:10000). This TNF alpha antibody is conjugated to ATTO 700. |
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Antibody for Human TNF alpha |
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| SPC-1315D-ALP | Stressmarq | 0.1ml | EUR 426 |
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Description: A polyclonal antibody for TNF alpha from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with Human Synthesized peptide derived from internal of human TNFA.. The Antibody is tested and validated for IHC, ICC/IF, ELISA assays with the following recommended dilutions: IHC (1:100), ICC/IF (1:500), ELISA (1:10000). This TNF alpha antibody is conjugated to Alkaline Phosphatase. |
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Antibody for Human TNF alpha |
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| SPC-1315D-APC | Stressmarq | 0.1ml | EUR 430.8 |
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Description: A polyclonal antibody for TNF alpha from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with Human Synthesized peptide derived from internal of human TNFA.. The Antibody is tested and validated for IHC, ICC/IF, ELISA assays with the following recommended dilutions: IHC (1:100), ICC/IF (1:500), ELISA (1:10000). This TNF alpha antibody is conjugated to APC . |
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Antibody for Human TNF alpha |
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| SPC-1315D-APCCY7 | Stressmarq | 0.1ml | EUR 518.4 |
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Description: A polyclonal antibody for TNF alpha from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with Human Synthesized peptide derived from internal of human TNFA.. The Antibody is tested and validated for IHC, ICC/IF, ELISA assays with the following recommended dilutions: IHC (1:100), ICC/IF (1:500), ELISA (1:10000). This TNF alpha antibody is conjugated to APC/Cy7. |
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Antibody for Human TNF alpha |
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| SPC-1315D-BI | Stressmarq | 0.1ml | EUR 428.4 |
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Description: A polyclonal antibody for TNF alpha from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with Human Synthesized peptide derived from internal of human TNFA.. The Antibody is tested and validated for IHC, ICC/IF, ELISA assays with the following recommended dilutions: IHC (1:100), ICC/IF (1:500), ELISA (1:10000). This TNF alpha antibody is conjugated to Biotin. |
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Antibody for Human TNF alpha |
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| SPC-1315D-DY350 | Stressmarq | 0.1ml | EUR 523.2 |
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Description: A polyclonal antibody for TNF alpha from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with Human Synthesized peptide derived from internal of human TNFA.. The Antibody is tested and validated for IHC, ICC/IF, ELISA assays with the following recommended dilutions: IHC (1:100), ICC/IF (1:500), ELISA (1:10000). This TNF alpha antibody is conjugated to Dylight 350. |
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Antibody for Human TNF alpha |
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| SPC-1315D-DY405 | Stressmarq | 0.1ml | EUR 494.4 |
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Description: A polyclonal antibody for TNF alpha from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with Human Synthesized peptide derived from internal of human TNFA.. The Antibody is tested and validated for IHC, ICC/IF, ELISA assays with the following recommended dilutions: IHC (1:100), ICC/IF (1:500), ELISA (1:10000). This TNF alpha antibody is conjugated to Dylight 405. |
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Antibody for Human TNF alpha |
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| SPC-1315D-DY488 | Stressmarq | 0.1ml | EUR 471.6 |
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Description: A polyclonal antibody for TNF alpha from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with Human Synthesized peptide derived from internal of human TNFA.. The Antibody is tested and validated for IHC, ICC/IF, ELISA assays with the following recommended dilutions: IHC (1:100), ICC/IF (1:500), ELISA (1:10000). This TNF alpha antibody is conjugated to Dylight 488. |
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Antibody for Human TNF alpha |
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| SPC-1315D-DY594 | Stressmarq | 0.1ml | EUR 476.4 |
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Description: A polyclonal antibody for TNF alpha from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with Human Synthesized peptide derived from internal of human TNFA.. The Antibody is tested and validated for IHC, ICC/IF, ELISA assays with the following recommended dilutions: IHC (1:100), ICC/IF (1:500), ELISA (1:10000). This TNF alpha antibody is conjugated to Dylight 594. |
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Antibody for Human TNF alpha |
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| SPC-1315D-DY633 | Stressmarq | 0.1ml | EUR 464.4 |
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Description: A polyclonal antibody for TNF alpha from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with Human Synthesized peptide derived from internal of human TNFA.. The Antibody is tested and validated for IHC, ICC/IF, ELISA assays with the following recommended dilutions: IHC (1:100), ICC/IF (1:500), ELISA (1:10000). This TNF alpha antibody is conjugated to Dylight 633. |
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Antibody for Human TNF alpha |
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| SPC-1315D-FITC | Stressmarq | 0.1ml | EUR 423.6 |
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Description: A polyclonal antibody for TNF alpha from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with Human Synthesized peptide derived from internal of human TNFA.. The Antibody is tested and validated for IHC, ICC/IF, ELISA assays with the following recommended dilutions: IHC (1:100), ICC/IF (1:500), ELISA (1:10000). This TNF alpha antibody is conjugated to FITC. |
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Antibody for Human TNF alpha |
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| SPC-1315D-HRP | Stressmarq | 0.1ml | EUR 418.8 |
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Description: A polyclonal antibody for TNF alpha from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with Human Synthesized peptide derived from internal of human TNFA.. The Antibody is tested and validated for IHC, ICC/IF, ELISA assays with the following recommended dilutions: IHC (1:100), ICC/IF (1:500), ELISA (1:10000). This TNF alpha antibody is conjugated to HRP. |
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Antibody for Human TNF alpha |
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| SPC-1315D-P594 | Stressmarq | 0.1ml | EUR 440.4 |
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Description: A polyclonal antibody for TNF alpha from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with Human Synthesized peptide derived from internal of human TNFA.. The Antibody is tested and validated for IHC, ICC/IF, ELISA assays with the following recommended dilutions: IHC (1:100), ICC/IF (1:500), ELISA (1:10000). This TNF alpha antibody is conjugated to PE/ATTO 594. |
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Antibody for Human TNF alpha |
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| SPC-1315D-PCP | Stressmarq | 0.1ml | EUR 430.8 |
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Description: A polyclonal antibody for TNF alpha from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with Human Synthesized peptide derived from internal of human TNFA.. The Antibody is tested and validated for IHC, ICC/IF, ELISA assays with the following recommended dilutions: IHC (1:100), ICC/IF (1:500), ELISA (1:10000). This TNF alpha antibody is conjugated to PerCP. |
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Antibody for Human TNF alpha |
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| SPC-1315D-RPE | Stressmarq | 0.1ml | EUR 429.6 |
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Description: A polyclonal antibody for TNF alpha from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with Human Synthesized peptide derived from internal of human TNFA.. The Antibody is tested and validated for IHC, ICC/IF, ELISA assays with the following recommended dilutions: IHC (1:100), ICC/IF (1:500), ELISA (1:10000). This TNF alpha antibody is conjugated to RPE . |
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Antibody for Human TNF alpha |
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| SPC-1315D-STR | Stressmarq | 0.1ml | EUR 430.8 |
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Description: A polyclonal antibody for TNF alpha from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with Human Synthesized peptide derived from internal of human TNFA.. The Antibody is tested and validated for IHC, ICC/IF, ELISA assays with the following recommended dilutions: IHC (1:100), ICC/IF (1:500), ELISA (1:10000). This TNF alpha antibody is conjugated to Streptavidin. |
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Rabbit Anti-Human alpha-Tubulin IgG |
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| TUBA13-A | Alpha Diagnostics | 100 ul | EUR 578.4 |
Recombinant (E.Coli) Human p38 alpha/SAPK2 alpha |
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| RP-675 | Alpha Diagnostics | 1 ug | EUR 343.2 |
Monoclonal Anti-Human Tumor Necrosis Factor-Alpha (TNF-alpha) IgG #1 aff. Pure |
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| TNFA11-M | Alpha Diagnostics | 100 ug | EUR 578.4 |
Monoclonal Anti-Human Tumor Necrosis Factor-Alpha (TNF-alpha) IgG #2, aff. Pure |
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| TNFA12-M | Alpha Diagnostics | 100 ug | EUR 578.4 |
Monoclonal Anti-Human alpha-Tubulin IgG |
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| TUBA12-M | Alpha Diagnostics | 100 ul | EUR 578.4 |
Rabbit Anti-Rat TNF-alpha Converting Enzyme (TACE) antiserum |
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| TACE11-S | Alpha Diagnostics | 100 ul | EUR 548.4 |
Human TNF alpha Protein |
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| abx060828-005mg | Abbexa | 0.05 mg | EUR 661.2 |
Alpha 2-Antiplasmin, Human Plasma |
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| A2AP15-100 | Alpha Diagnostics | 100 ug | EUR 196.8 |
Alpha 1-Antitrypsin, Human Plasma |
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| A1AT15-N | Alpha Diagnostics | 1 mg | EUR 196.8 |
Chicken Anti-Human alpha 1 anti-trypsin IgG/Y |
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| A1AT12-A | Alpha Diagnostics | 100 ul | EUR 578.4 |
Rabbit Anti-Human p73 alpha antiserum # 2 |
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| P73A12-S | Alpha Diagnostics | 100 ul | EUR 548.4 |
Guinea pig Tumor Necrosis Factor Alpha (TNF-a) ELISA Kit, 96 tests, Quantitative |
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| 9870 | Alpha Diagnostics | 1 Kit | EUR 927.6 |
Tumor necrosis factor alpha TNF-? (72 - 82), human (AA: Pro-Leu-Ala-Gln-Ala-Val-Arg-Ser-Ser-Ser-Arg) (MW: 1171.33) |
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| SP-86635-5 | Alpha Diagnostics | 5 mg | EUR 343.2 |
Rabbit Anti-Human Glutathione Transferase alpha (GST-alpha) antiserum |
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| GSTA12-S | Alpha Diagnostics | 100 ul | EUR 548.4 |
Alpha 1-Antichymotrypsin , Human Plasma |
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| A1CT15-N-100 | Alpha Diagnostics | 100 ug | EUR 196.8 |
Goat Anti-Human Tumor Necrosis Factor-Alpha (TNF-alpha) IgG #2, aff. Pure (neutralzing) |
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| TNFA13-A | Alpha Diagnostics | 100 ul | EUR 578.4 |
Rabbit Anti-Human Estrogen receptor alpha (ER-alpha) peptide IgG |
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| ERA12-A | Alpha Diagnostics | 100 ug | EUR 578.4 |
Rabbit Anti-Human p73 alpha/beta antiserum # 1 |
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| P73A11-S | Alpha Diagnostics | 100 ul | EUR 548.4 |
Monoclonal anti-human Alpha (FSH-Alpha), IgG clone 3 aff pure |
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| FSHA12-M | Alpha Diagnostics | 1 mg | EUR 578.4 |
Anti-mouse TNF alpha Antibody |
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| RP1000 | BosterBio | 100ug/vial | EUR 400.8 |
Goat Anti-Human Lutenizing Hormone Alpha (LH-Alpha) IgG aff pure |
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| LHA13-A | Alpha Diagnostics | 1 mg | EUR 343.2 |
human Topoisomerase II alpha (TOP2 alpha) Control/blocking peptide |
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| TOP2A11-P | Alpha Diagnostics | 100 ug | EUR 196.8 |
Goat Anti-human Alpha fetoprotein (AFP) IgG, pure |
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| AFP13-A | Alpha Diagnostics | 1 mg | EUR 781.2 |
Human TNF alpha ELISA kit |
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| 55R-IB49623 | Fitzgerald | 96 wells | EUR 1413.6 |
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Description: ELISA kit for the detection of TNF alpha in the research laboratory |
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Human TNF alpha ELISA kit |
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| 55R-1690 | Fitzgerald | 1 kit | EUR 481.2 |
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Description: ELISA kit for the detection of TNF alpha in the research laboratory |
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Goat Anti-human Chorionic Gonadotropin Alpha (hCG-Alpha) IgG aff pure |
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| HCGA13-A | Alpha Diagnostics | 1 mg | EUR 343.2 |
Rabbit Anti-Human p73 alpha/ IgG # 2, aff pure |
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| P73A12-A | Alpha Diagnostics | 100 ug | EUR 578.4 |
Rabbit Anti-Human HE2 alpha IgG #1, aff pure |
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| HE2A11-A | Alpha Diagnostics | 100 ug | EUR 578.4 |
Human TNF Alpha (TNFA) Protein |
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| 20-abx670178 | Abbexa |
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Recombinant (E.Coli) purified human Tumor Necrosis Factor-Alpha (TNF-alpha), biologically active |
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| TNFA15-R-10 | Alpha Diagnostics | 10 ug | EUR 270 |
Recombinant purified, human Interleukin-1 alpha (IL-1 alpha), active |
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| IL1A15-R-10 | Alpha Diagnostics | 10 ug | EUR 416.4 |
Rabbit Anti-Human alpha-Tubulin IgG-HRP Conjugate |
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| TUBA13-HRP | Alpha Diagnostics | 100 ul | EUR 518.4 |
Recombinant Human Alpha-Synuclein |
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| RP-847 | Alpha Diagnostics | 20 ug | EUR 196.8 |
Tumor necrosis factor alpha TNF-? (78-96) (human) (AA: His-Thr-Ile-Ser-Arg-Ile-Ala-Val-Ser-Tyr-Gln-Thr-Lys-Val-Asn-Leu-Leu-Ser-Ala) (MW: 2101.45) |
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| SP-89730-1 | Alpha Diagnostics | 1 mg | EUR 196.8 |
Goat Anti-Ferret IgA (alpha), unlabeled |
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| 70519 | Alpha Diagnostics | 0.5 mg | EUR 255.6 |
Rabbit Anti-Human alpha-Tubulin IgG-Biotin Conjugate |
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| TUBA13-BTN | Alpha Diagnostics | 100 ul | EUR 562.8 |
Human Alpha-Thrombin (human plasma), purified |
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| TBNA16-N-100 | Alpha Diagnostics | 100 ug | EUR 196.8 |
Tumor necrosis factor alpha TNF-? (46-65) (human) (AA: Asn-Gln-Leu-Val-Val-Pro-Ser-Glu-Gly-Leu-Tyr-Leu-Ile-Tyr-Ser-Gln-Val-Leu-Phe-Lys) (MW: 2310.74) |
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| SP-89728-1 | Alpha Diagnostics | 1 mg | EUR 489.6 |
Rabbit Anti-Rat TNF-alpha Converting Enzyme (TACE), IgG aff. Pure |
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| TACE11-A | Alpha Diagnostics | 100 ug | EUR 578.4 |
Rabbit Anti-Human Resistin-Like protein alpha (RELM-alpha/FIZZ1) antiserum #2 |
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| RELMA12-S | Alpha Diagnostics | 100 ul | EUR 548.4 |
Goat Anti-Human Alpha 1-Antitrypsin (A1AT) IgG, unlabeled |
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| A1AT11-A | Alpha Diagnostics | 100 ul | EUR 489.6 |
Chicken Anti-Human Alpha 2 Macroglobulin (A2M) IgG/Y |
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| A2MG13-A | Alpha Diagnostics | 100 ul | EUR 578.4 |
Rabbit Anti-Rat Glutathione Transferase alpha (GST-alpha) antiserum |
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| GSTA11-S | Alpha Diagnostics | 100 ul | EUR 548.4 |
TNF alpha |
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| GT15064 | Neuromics | 100 ug | EUR 631.2 |
TNF alpha |
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| GT15113 | Neuromics | 100 ug | EUR 631.2 |
Rabbit Anti-Human p73 alpha/beta IgG # 1, aff pure |
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| P73A11-A | Alpha Diagnostics | 100 ug | EUR 578.4 |
Tumor necrosis factor alpha TNF-? (31 - 45), human (AA: Arg-Arg-Ala-Asn-Ala-Leu-Leu-Ala-Asn-Gly-Val-Glu-Leu-Arg-Asp) (MW: 1667.90) |
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| SP-86636-1 | Alpha Diagnostics | 1 mg | EUR 196.8 |
anti-TNF alpha |
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| YF-PA24865 | Abfrontier | 50 ul | EUR 400.8 |
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Description: Mouse polyclonal to TNF alpha |
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Monoclonal Anti-Human Chorionic Gonadotropin Alpha (hCG-alpha) IgG #1, aff pure |
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| HCGA11-M | Alpha Diagnostics | 1 mg | EUR 343.2 |
TNF alpha antibody |
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| 10-T60A | Fitzgerald | 1 mg | EUR 159.6 |
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Description: Mouse monoclonal TNF alpha antibody |
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TNF alpha antibody |
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| 10-T60B | Fitzgerald | 1 mg | EUR 536.4 |
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Description: Mouse monoclonal TNF alpha antibody |
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Filling the Antibody Pipeline in Allergy: PIPE Cloning of IgE, IgG 1 and IgG 4 against the Major Birch Pollen Allergen Bet v 1
Birch pollen allergy is among the most prevalent pollen allergies in Northern and Central Europe. This IgE-mediated disease can be treated with allergen immunotherapy (AIT), which typically gives rise to IgG antibodies inducing tolerance. Although the main mechanisms of allergen immunotherapy (AIT) are known, questions regarding possible Fc-mediated effects of IgG antibodies remain unanswered.
This can mainly be attributed to the unavailability of appropriate tools, i.e., well-characterised recombinant antibodies (rAbs). We hereby aimed at providing human rAbs of several classes for mechanistic studies and as possible candidates for passive immunotherapy.
We engineered IgE, IgG1, and IgG4 sharing the same variable region against the major birch pollen allergen Bet v 1 using Polymerase Incomplete Primer Extension (PIPE) cloning. We tested IgE functionality and IgG blocking capabilities using appropriate model cell lines.
In vitro studies showed IgE engagement with FcεRI and CD23 and Bet v 1-dependent degranulation. Overall, we hereby present fully functional, human IgE, IgG1, and IgG4 sharing the same variable region against Bet v 1 and showcase possible applications in first mechanistic studies.
Furthermore, our IgG antibodies might be useful candidates for passive immunotherapy of birch pollen allergy.
LEC Chemokine(LEC67) Antibody |
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| BNC800067-500 | Biotium | 500uL | EUR 652.8 |
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Description: Primary antibody against LEC Chemokine(LEC67), CF680 conjugate, Concentration: 0.1mg/mL |
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LEC Chemokine(LEC66) Antibody |
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| BNC810066-100 | Biotium | 100uL | EUR 238.8 |
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Description: Primary antibody against LEC Chemokine(LEC66), CF680R conjugate, Concentration: 0.1mg/mL |
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LEC Chemokine(LEC66) Antibody |
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| BNC810066-500 | Biotium | 500uL | EUR 652.8 |
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Description: Primary antibody against LEC Chemokine(LEC66), CF680R conjugate, Concentration: 0.1mg/mL |
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LEC Chemokine(LEC67) Antibody |
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| BNC810067-100 | Biotium | 100uL | EUR 238.8 |
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Description: Primary antibody against LEC Chemokine(LEC67), CF680R conjugate, Concentration: 0.1mg/mL |
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LEC Chemokine(LEC67) Antibody |
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| BNC810067-500 | Biotium | 500uL | EUR 652.8 |
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Description: Primary antibody against LEC Chemokine(LEC67), CF680R conjugate, Concentration: 0.1mg/mL |
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LEC Chemokine(LEC66) Antibody |
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| BNC940066-100 | Biotium | 100uL | EUR 238.8 |
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Description: Primary antibody against LEC Chemokine(LEC66), CF594 conjugate, Concentration: 0.1mg/mL |
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LEC Chemokine(LEC66) Antibody |
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| BNC940066-500 | Biotium | 500uL | EUR 652.8 |
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Description: Primary antibody against LEC Chemokine(LEC66), CF594 conjugate, Concentration: 0.1mg/mL |
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LEC Chemokine(LEC67) Antibody |
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| BNC940067-100 | Biotium | 100uL | EUR 238.8 |
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Description: Primary antibody against LEC Chemokine(LEC67), CF594 conjugate, Concentration: 0.1mg/mL |
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LEC Chemokine(LEC67) Antibody |
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| BNC940067-500 | Biotium | 500uL | EUR 652.8 |
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Description: Primary antibody against LEC Chemokine(LEC67), CF594 conjugate, Concentration: 0.1mg/mL |
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LEC Chemokine(LEC66) Antibody |
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| BNC880066-100 | Biotium | 100uL | EUR 238.8 |
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Description: Primary antibody against LEC Chemokine(LEC66), CF488A conjugate, Concentration: 0.1mg/mL |
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LEC Chemokine(LEC66) Antibody |
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| BNC880066-500 | Biotium | 500uL | EUR 652.8 |
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Description: Primary antibody against LEC Chemokine(LEC66), CF488A conjugate, Concentration: 0.1mg/mL |
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LEC Chemokine(LEC67) Antibody |
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| BNC880067-100 | Biotium | 100uL | EUR 238.8 |
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Description: Primary antibody against LEC Chemokine(LEC67), CF488A conjugate, Concentration: 0.1mg/mL |
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LEC Chemokine(LEC67) Antibody |
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| BNC880067-500 | Biotium | 500uL | EUR 652.8 |
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Description: Primary antibody against LEC Chemokine(LEC67), CF488A conjugate, Concentration: 0.1mg/mL |
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LEC Chemokine(LEC66) Antibody |
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| BNC430066-100 | Biotium | 100uL | EUR 238.8 |
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Description: Primary antibody against LEC Chemokine(LEC66), CF543 conjugate, Concentration: 0.1mg/mL |
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LEC Chemokine(LEC66) Antibody |
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| BNC430066-500 | Biotium | 500uL | EUR 652.8 |
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Description: Primary antibody against LEC Chemokine(LEC66), CF543 conjugate, Concentration: 0.1mg/mL |
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LEC Chemokine(LEC67) Antibody |
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| BNC430067-100 | Biotium | 100uL | EUR 238.8 |
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Description: Primary antibody against LEC Chemokine(LEC67), CF543 conjugate, Concentration: 0.1mg/mL |
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LEC Chemokine(LEC67) Antibody |
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| BNC430067-500 | Biotium | 500uL | EUR 652.8 |
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Description: Primary antibody against LEC Chemokine(LEC67), CF543 conjugate, Concentration: 0.1mg/mL |
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LEC Chemokine(LEC66) Antibody |
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| BNC400066-100 | Biotium | 100uL | EUR 238.8 |
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Description: Primary antibody against LEC Chemokine(LEC66), CF640R conjugate, Concentration: 0.1mg/mL |
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LEC Chemokine(LEC66) Antibody |
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| BNC400066-500 | Biotium | 500uL | EUR 652.8 |
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Description: Primary antibody against LEC Chemokine(LEC66), CF640R conjugate, Concentration: 0.1mg/mL |
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LEC Chemokine(LEC67) Antibody |
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| BNC400067-100 | Biotium | 100uL | EUR 238.8 |
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Description: Primary antibody against LEC Chemokine(LEC67), CF640R conjugate, Concentration: 0.1mg/mL |
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LEC Chemokine(LEC67) Antibody |
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| BNC400067-500 | Biotium | 500uL | EUR 652.8 |
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Description: Primary antibody against LEC Chemokine(LEC67), CF640R conjugate, Concentration: 0.1mg/mL |
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LEC Chemokine(LEC66) Antibody |
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| BNC050066-100 | Biotium | 100uL | EUR 238.8 |
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Description: Primary antibody against LEC Chemokine(LEC66), CF405M conjugate, Concentration: 0.1mg/mL |
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LEC Chemokine(LEC66) Antibody |
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| BNC050066-500 | Biotium | 500uL | EUR 652.8 |
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Description: Primary antibody against LEC Chemokine(LEC66), CF405M conjugate, Concentration: 0.1mg/mL |
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LEC Chemokine(LEC67) Antibody |
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| BNC050067-100 | Biotium | 100uL | EUR 238.8 |
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Description: Primary antibody against LEC Chemokine(LEC67), CF405M conjugate, Concentration: 0.1mg/mL |
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LEC Chemokine(LEC67) Antibody |
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| BNC050067-500 | Biotium | 500uL | EUR 652.8 |
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Description: Primary antibody against LEC Chemokine(LEC67), CF405M conjugate, Concentration: 0.1mg/mL |
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LEC Chemokine(LEC66) Antibody |
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| BNCAP0066-100 | Biotium | 100uL | EUR 238.8 |
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Description: Primary antibody against LEC Chemokine(LEC66), Alkaline Phosphatase conjugate, Concentration: 0.1mg/mL |
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LEC Chemokine(LEC66) Antibody |
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| BNCAP0066-500 | Biotium | 500uL | EUR 652.8 |
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Description: Primary antibody against LEC Chemokine(LEC66), Alkaline Phosphatase conjugate, Concentration: 0.1mg/mL |
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LEC Chemokine(LEC67) Antibody |
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| BNCAP0067-100 | Biotium | 100uL | EUR 238.8 |
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Description: Primary antibody against LEC Chemokine(LEC67), Alkaline Phosphatase conjugate, Concentration: 0.1mg/mL |
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LEC Chemokine(LEC67) Antibody |
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| BNCAP0067-500 | Biotium | 500uL | EUR 652.8 |
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Description: Primary antibody against LEC Chemokine(LEC67), Alkaline Phosphatase conjugate, Concentration: 0.1mg/mL |
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LEC Chemokine(LEC66) Antibody |
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| BNCB0066-100 | Biotium | 100uL | EUR 238.8 |
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Description: Primary antibody against LEC Chemokine(LEC66), Biotin conjugate, Concentration: 0.1mg/mL |
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LEC Chemokine(LEC66) Antibody |
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| BNCB0066-500 | Biotium | 500uL | EUR 652.8 |
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Description: Primary antibody against LEC Chemokine(LEC66), Biotin conjugate, Concentration: 0.1mg/mL |
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LEC Chemokine(LEC67) Antibody |
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| BNCB0067-100 | Biotium | 100uL | EUR 238.8 |
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Description: Primary antibody against LEC Chemokine(LEC67), Biotin conjugate, Concentration: 0.1mg/mL |
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LEC Chemokine(LEC67) Antibody |
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| BNCB0067-500 | Biotium | 500uL | EUR 652.8 |
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Description: Primary antibody against LEC Chemokine(LEC67), Biotin conjugate, Concentration: 0.1mg/mL |
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LEC Chemokine(LEC66) Antibody |
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| BNCR0066-250 | Biotium | 250uL | EUR 459.6 |
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Description: Primary antibody against LEC Chemokine(LEC66), RPE conjugate, Concentration: 0.1mg/mL |
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LEC Chemokine(LEC67) Antibody |
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| BNCR0067-250 | Biotium | 250uL | EUR 459.6 |
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Description: Primary antibody against LEC Chemokine(LEC67), RPE conjugate, Concentration: 0.1mg/mL |
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LEC Chemokine(LEC66) Antibody |
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| BNUB0066-100 | Biotium | 100uL | EUR 250.8 |
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Description: Primary antibody against LEC Chemokine(LEC66), Concentration: 0.2mg/mL |
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LEC Chemokine(LEC66) Antibody |
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| BNUB0066-500 | Biotium | 500uL | EUR 549.6 |
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Description: Primary antibody against LEC Chemokine(LEC66), Concentration: 0.2mg/mL |
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LEC Chemokine(LEC67) Antibody |
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| BNUB0067-100 | Biotium | 100uL | EUR 250.8 |
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Description: Primary antibody against LEC Chemokine(LEC67), Concentration: 0.2mg/mL |
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LEC Chemokine(LEC67) Antibody |
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| BNUB0067-500 | Biotium | 500uL | EUR 549.6 |
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Description: Primary antibody against LEC Chemokine(LEC67), Concentration: 0.2mg/mL |
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LEC Chemokine(LEC66) Antibody |
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| BNCP0066-250 | Biotium | 250uL | EUR 459.6 |
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Description: Primary antibody against LEC Chemokine(LEC66), PerCP conjugate, Concentration: 0.1mg/mL |
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LEC Chemokine(LEC67) Antibody |
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| BNCP0067-250 | Biotium | 250uL | EUR 459.6 |
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Description: Primary antibody against LEC Chemokine(LEC67), PerCP conjugate, Concentration: 0.1mg/mL |
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LEC Chemokine(LEC66) Antibody |
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| BNUM0066-50 | Biotium | 50uL | EUR 474 |
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Description: Primary antibody against LEC Chemokine(LEC66), 1mg/mL |
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LEC Chemokine(LEC67) Antibody |
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| BNUM0067-50 | Biotium | 50uL | EUR 474 |
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Description: Primary antibody against LEC Chemokine(LEC67), 1mg/mL |
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LEC Chemokine(LEC66) Antibody |
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| BNCH0066-100 | Biotium | 100uL | EUR 238.8 |
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Description: Primary antibody against LEC Chemokine(LEC66), Horseradish Peroxidase conjugate, Concentration: 0.1mg/mL |
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LEC Chemokine(LEC66) Antibody |
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| BNCH0066-500 | Biotium | 500uL | EUR 652.8 |
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Description: Primary antibody against LEC Chemokine(LEC66), Horseradish Peroxidase conjugate, Concentration: 0.1mg/mL |
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LEC Chemokine(LEC67) Antibody |
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| BNCH0067-100 | Biotium | 100uL | EUR 238.8 |
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Description: Primary antibody against LEC Chemokine(LEC67), Horseradish Peroxidase conjugate, Concentration: 0.1mg/mL |
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LEC Chemokine(LEC67) Antibody |
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| BNCH0067-500 | Biotium | 500uL | EUR 652.8 |
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Description: Primary antibody against LEC Chemokine(LEC67), Horseradish Peroxidase conjugate, Concentration: 0.1mg/mL |
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LEC Chemokine(LEC66) Antibody |
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| BNC040066-100 | Biotium | 100uL | EUR 238.8 |
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Description: Primary antibody against LEC Chemokine(LEC66), CF405S conjugate, Concentration: 0.1mg/mL |
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LEC Chemokine(LEC66) Antibody |
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| BNC040066-500 | Biotium | 500uL | EUR 652.8 |
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Description: Primary antibody against LEC Chemokine(LEC66), CF405S conjugate, Concentration: 0.1mg/mL |
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LEC Chemokine(LEC67) Antibody |
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| BNC040067-100 | Biotium | 100uL | EUR 238.8 |
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Description: Primary antibody against LEC Chemokine(LEC67), CF405S conjugate, Concentration: 0.1mg/mL |
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LEC Chemokine(LEC67) Antibody |
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| BNC040067-500 | Biotium | 500uL | EUR 652.8 |
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Description: Primary antibody against LEC Chemokine(LEC67), CF405S conjugate, Concentration: 0.1mg/mL |
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LEC Chemokine(LEC66) Antibody |
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| BNC550066-100 | Biotium | 100uL | EUR 238.8 |
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Description: Primary antibody against LEC Chemokine(LEC66), CF555 conjugate, Concentration: 0.1mg/mL |
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LEC Chemokine(LEC66) Antibody |
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| BNC550066-500 | Biotium | 500uL | EUR 652.8 |
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Description: Primary antibody against LEC Chemokine(LEC66), CF555 conjugate, Concentration: 0.1mg/mL |
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LEC Chemokine(LEC67) Antibody |
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| BNC550067-100 | Biotium | 100uL | EUR 238.8 |
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Description: Primary antibody against LEC Chemokine(LEC67), CF555 conjugate, Concentration: 0.1mg/mL |
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LEC Chemokine(LEC67) Antibody |
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| BNC550067-500 | Biotium | 500uL | EUR 652.8 |
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Description: Primary antibody against LEC Chemokine(LEC67), CF555 conjugate, Concentration: 0.1mg/mL |
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LEC Chemokine(LEC66) Antibody |
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| BNC610066-100 | Biotium | 100uL | EUR 238.8 |
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Description: Primary antibody against LEC Chemokine(LEC66), CF660R conjugate, Concentration: 0.1mg/mL |
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LEC Chemokine(LEC66) Antibody |
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| BNC610066-500 | Biotium | 500uL | EUR 652.8 |
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Description: Primary antibody against LEC Chemokine(LEC66), CF660R conjugate, Concentration: 0.1mg/mL |
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LEC Chemokine(LEC67) Antibody |
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| BNC610067-100 | Biotium | 100uL | EUR 238.8 |
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Description: Primary antibody against LEC Chemokine(LEC67), CF660R conjugate, Concentration: 0.1mg/mL |
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LEC Chemokine(LEC67) Antibody |
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| BNC610067-500 | Biotium | 500uL | EUR 652.8 |
|
Description: Primary antibody against LEC Chemokine(LEC67), CF660R conjugate, Concentration: 0.1mg/mL |
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LEC Chemokine(LEC66) Antibody |
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| BNC680066-100 | Biotium | 100uL | EUR 238.8 |
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Description: Primary antibody against LEC Chemokine(LEC66), CF568 conjugate, Concentration: 0.1mg/mL |
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LEC Chemokine(LEC66) Antibody |
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| BNC680066-500 | Biotium | 500uL | EUR 652.8 |
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Description: Primary antibody against LEC Chemokine(LEC66), CF568 conjugate, Concentration: 0.1mg/mL |
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LEC Chemokine(LEC67) Antibody |
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| BNC680067-100 | Biotium | 100uL | EUR 238.8 |
|
Description: Primary antibody against LEC Chemokine(LEC67), CF568 conjugate, Concentration: 0.1mg/mL |
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LEC Chemokine(LEC67) Antibody |
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| BNC680067-500 | Biotium | 500uL | EUR 652.8 |
|
Description: Primary antibody against LEC Chemokine(LEC67), CF568 conjugate, Concentration: 0.1mg/mL |
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LEC Chemokine(LEC66) Antibody |
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| BNC470066-100 | Biotium | 100uL | EUR 238.8 |
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Description: Primary antibody against LEC Chemokine(LEC66), CF647 conjugate, Concentration: 0.1mg/mL |
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LEC Chemokine(LEC66) Antibody |
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| BNC470066-500 | Biotium | 500uL | EUR 652.8 |
|
Description: Primary antibody against LEC Chemokine(LEC66), CF647 conjugate, Concentration: 0.1mg/mL |
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LEC Chemokine(LEC67) Antibody |
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| BNC470067-100 | Biotium | 100uL | EUR 238.8 |
|
Description: Primary antibody against LEC Chemokine(LEC67), CF647 conjugate, Concentration: 0.1mg/mL |
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LEC Chemokine(LEC67) Antibody |
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| BNC470067-500 | Biotium | 500uL | EUR 652.8 |
|
Description: Primary antibody against LEC Chemokine(LEC67), CF647 conjugate, Concentration: 0.1mg/mL |
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LEC Chemokine(LEC66) Antibody |
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| BNC700066-100 | Biotium | 100uL | EUR 238.8 |
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Description: Primary antibody against LEC Chemokine(LEC66), CF770 conjugate, Concentration: 0.1mg/mL |
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LEC Chemokine(LEC66) Antibody |
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| BNC700066-500 | Biotium | 500uL | EUR 652.8 |
|
Description: Primary antibody against LEC Chemokine(LEC66), CF770 conjugate, Concentration: 0.1mg/mL |
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LEC Chemokine(LEC67) Antibody |
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| BNC700067-100 | Biotium | 100uL | EUR 238.8 |
|
Description: Primary antibody against LEC Chemokine(LEC67), CF770 conjugate, Concentration: 0.1mg/mL |
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LEC Chemokine(LEC67) Antibody |
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| BNC700067-500 | Biotium | 500uL | EUR 652.8 |
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Description: Primary antibody against LEC Chemokine(LEC67), CF770 conjugate, Concentration: 0.1mg/mL |
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LEC protein |
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| 30R-AL013 | Fitzgerald | 20 ug | EUR 327.6 |
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Description: Purified recombinant Human LEC protein |
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