PARPtrap™ Combo Assay Kit for PARP1 and PARP2 |
78317 |
BPS Bioscience |
384 rxns. |
EUR 3000 |
Description: The PARPtrap™ Combo Assay Kit for PARP1 and PARP2 is a kit designed to measure DNA complex formations of PARP1 and PARP2 enzymes in a high throughput screening assay using fluorescence polarization (FP). The key to the PARPtrap™ Combo Assay Kit are the fluorescent-labeled DNA probes recognized by PARP1 and PARP2. In the absence of ribosylation, PARP1/2 binds to the DNA fluorescent probe, forming a large complex and resulting in the emission of highly polarized light. However, after autoribosylation, PARP1/2 dissociates from the oligonucleotide duplex, which then rotates freely, emitting less polarized light (Fig. 1). Addition of a PARP inhibitor results in trapping of PARP1/2 to the fluorescent-labeled DNA, and increases the FP signal in a dose dependent manner.The combo kit is particularly useful to directly and specifically compare, in a single assay, the effect and potency of a compound on PARP1 and PARP2. The PARPtrap™ Combo Assay Kit for PARP1 and PARP2 is a homogeneous fluorescence polarization assay. The FP signal is measured using a fluorescent microplate reader capable of measuring fluorescence polarization. |
Bnp Assay Laboratories manufactures the bnp assay is for reagents distributed by Genprice. The Bnp Assay Is For reagent is RUO (Research Use Only) to test human serum or cell culture lab samples. To purchase these products, for the MSDS, Data Sheet, protocol, storage conditions/temperature or for the concentration, please contact bnp assay. Other Bnp products are available in stock. Specificity: Bnp Category: Assay Group: Is For
PARPtrap™ Assay Kit for PARP2 |
BPS Bioscience |
384 rxns. |
EUR 3070 |
Description: The PARPtrap™ Assay Kit for PARP2 is designed to measure PARP2/DNA complex formation in a high throughput screening assay using fluorescence polarization (FP). Similarly, to PARP1, PARP2 recognizes and binds damaged DNA via its DNA-binding domain. Binding to DNA activates PARP2 and in the presence of NAD+ PARP2 ribosylates itself (auto-ribosylation), which leads to PARP2 dissociation from the DNA due to the accumulated negative charge of the ribosyl polymer. In the presence of some inhibitors, however, PARP remains bound to the DNA, a phenomenon termed trapping. Trapped PARP-DNA complexes have been shown to be highly cytotoxic to cancer cells, therefore such inhibitors may be desirable for cancer treatment. The key to the PARPtrap™ Assay Kit is the fluorescent-labeled oligonucleotide duplex. In the absence of ribosylation, PARP2 binds to the fluorescent probe, forming a large complex and resulting in the emission of highly polarized light. However, after auto-ribosylation PARP2 dissociates from the oligonucleotide duplex, which then rotates freely, emitting less polarized light (Fig. 1). Addition of a PARP2 inhibitor results in trapping of PARP2 to the fluorescent oligonucleotide duplex, and increases the FP signal in a dose dependent manner.The PARPtrap™ Assay Kit is a fluorescence polarization homogeneous assay. The FP signal is measured using a fluorescent microplate reader capable of measuring fluorescence polarization. |
Glycerol Assay Kit (For liquid samples) |
Abbexa |
1 Kit |
EUR 735.6 |
|
PARPtrap™ Assay Kit for PARP1 |
BPS Bioscience |
96 rxns. |
EUR 2145 |
Description: The PARPtrap™ Assay Kit for PARP1 is designed to measure PARP1/DNA complex formation in a high throughput screening assay using fluorescence polarization (FP). PARP1 is known to bind damaged DNA through its DNA-binding domains. Binding to DNA activates PARP1 and in the presence of NAD+ PARP1 ribosylates itself (auto-ribosylation), what in consequence leads to PARP1 dissociation from the DNA due to the accumulated negative charge of the ribosyl polymer. In the presence of some inhibitors, however, PARP remains bound to the DNA, a phenomenon termed trapping. Trapped PARP-DNA complexes have been shown to be highly cytotoxic to cancer cells, therefore such inhibitors may be desirable for cancer treatment.The key to the PARPtrap™ Assay Kit for PARP1 is the fluorescent-labeled oligonucleotide duplex. In the absence of ribosylation, PARP1 binds to the fluorescent probe, forming a large complex and resulting in the emission of highly polarized light. However, after auto-ribosylation, PARP1 dissociates from the oligonucleotide duplex, which then rotates freely, emitting less polarized light (Fig. 1). Addition of a PARP1 inhibitor results in trapping of PARP1 to the fluorescent oligonucleotide duplex, and increases the FP signal in a dose dependent manner.The PARPtrap™ Assay Kit for PARP1 is a fluorescence polarization homogeneous assay. The FP signal is measured using a fluorescent microplate reader capable of measuring fluorescence polarization. |
PARPtrap™ Assay Kit for PARP1 |
BPS Bioscience |
384 rxns. |
EUR 3070 |
Description: The PARPtrap™ Assay Kit for PARP1 is designed to measure PARP1/DNA complex formation in a high throughput screening assay using fluorescence polarization (FP). PARP1 is known to bind damaged DNA through its DNA-binding domains. Binding to DNA activates PARP1 and in the presence of NAD+ PARP1 ribosylates itself (auto-ribosylation), what in consequence leads to PARP1 dissociation from the DNA due to the accumulated negative charge of the ribosyl polymer. In the presence of some inhibitors, however, PARP remains bound to the DNA, a phenomenon termed trapping. Trapped PARP-DNA complexes have been shown to be highly cytotoxic to cancer cells, therefore such inhibitors may be desirable for cancer treatment.The key to the PARPtrap™ Assay Kit for PARP1 is the fluorescent-labeled oligonucleotide duplex. In the absence of ribosylation, PARP1 binds to the fluorescent probe, forming a large complex and resulting in the emission of highly polarized light. However, after auto-ribosylation, PARP1 dissociates from the oligonucleotide duplex, which then rotates freely, emitting less polarized light (Fig. 1). Addition of a PARP1 inhibitor results in trapping of PARP1 to the fluorescent oligonucleotide duplex, and increases the FP signal in a dose dependent manner.The PARPtrap™ Assay Kit for PARP1 is a fluorescence polarization homogeneous assay. The FP signal is measured using a fluorescent microplate reader capable of measuring fluorescence polarization. |
DPA/Terbium for membrane fusion assay |
Biotium |
KT |
EUR 92 |
SDIP/Europium for membrane fusion assay |
Biotium |
KT |
EUR 151 |
AOPI Viability Assay for Flow Cytometry |
Tribioscience |
5 mL |
EUR 98 |
Is For information
Rat Brain Natriuretic Peptide 32 (BNP-32) AssayLite Antibody (APC Conjugate) |
10541-05061 |
AssayPro |
150 ug |
EUR 513.6 |
Rat Brain Natriuretic Peptide 32 (BNP-32) AssayLite Antibody (PerCP Conjugate) |
10541-05071 |
AssayPro |
150 ug |
EUR 565.2 |
Streptavidin-HRP (For PARP & Cytokine Assay Kits) |
80611 |
BPS Bioscience |
100 µl |
EUR 100 |
Description: Streptavidin-HRP is useful in select BPS Bioscience assay kits. |
Micrococcus lysodeikticus, ATCC No.4698 for Lysozyme Assay |
23372-14 |
NACALAI TESQUE |
5G |
EUR 92.4 |
Hyperactive Universal CUT&Tag Assay Kit for Illumina |
TD903-01 |
Vazyme |
24 rxns |
EUR 300 |
Hyperactive Universal CUT&Tag Assay Kit for Illumina |
TD903-02 |
Vazyme |
48 rxns |
EUR 1114 |
QuDye dsDNA HS Assay kit (optimized for Qubit), 100 |
13102 |
Lumiprobe |
100 assays |
EUR 98.4 |
PARPtrap™ Combo Assay Kit for PARP1 and PARP2 |
78317 |
BPS Bioscience |
384 rxns. |
EUR 3000 |
Description: The PARPtrap™ Combo Assay Kit for PARP1 and PARP2 is a kit designed to measure DNA complex formations of PARP1 and PARP2 enzymes in a high throughput screening assay using fluorescence polarization (FP). The key to the PARPtrap™ Combo Assay Kit are the fluorescent-labeled DNA probes recognized by PARP1 and PARP2. In the absence of ribosylation, PARP1/2 binds to the DNA fluorescent probe, forming a large complex and resulting in the emission of highly polarized light. However, after autoribosylation, PARP1/2 dissociates from the oligonucleotide duplex, which then rotates freely, emitting less polarized light (Fig. 1). Addition of a PARP inhibitor results in trapping of PARP1/2 to the fluorescent-labeled DNA, and increases the FP signal in a dose dependent manner.The combo kit is particularly useful to directly and specifically compare, in a single assay, the effect and potency of a compound on PARP1 and PARP2. The PARPtrap™ Combo Assay Kit for PARP1 and PARP2 is a homogeneous fluorescence polarization assay. The FP signal is measured using a fluorescent microplate reader capable of measuring fluorescence polarization. |
Viability/Cytotoxity Assay Kit for Animal Live & Dead Cell (150 assays) |
30002-T |
Biotium |
1KIT |
EUR 219.6 |
Description: Minimum order quantity: 1 unit of 1KIT |
Viability/Cytotoxity Assay Kit for Animal Live & Dead Cell (150 assays) |
30002-T-1 |
Biotium |
KT |
EUR 150 |
Viability/Cytotoxity Assay Kit for Animal Live & Dead Cells (300 assays) |
30002-1 |
Biotium |
KT |
EUR 298 |
Pyrophosphate Assay Reagent for RNAP (enzyme not included) |
PPA1000 |
ProFoldin |
1000 assays |
EUR 372.78 |
Description: This product includes 3200 ul of Buffer RP, 310 ul of 100 x NTP mix, 310 ul of 100 x DNA, 32 ul of 1000 x pyrophosphatase and 42 ml of dye for 100 assays of E. coli RNA polymerase reactions in a 384-well assay format. The assay This product includes all reagents except the enzyme. |
Anti-Cetuximab Antibody (AY27) (recommended for ADA assay) |
CEB-Y27 |
ACROBIOSYSTEMS |
100ug |
EUR 2140 |
|
Description: Mouse monoclonal antibody is produced from a hybridoma resulting from fusion of SP2/0 myeloma and B-lymphocytes obtained from a mouse immunized with Cetuximab F(ab')2. |
Anti-Rituximab Antibody (AY36) (recommended for ADA assay) |
RIB-Y36 |
ACROBIOSYSTEMS |
100ug |
EUR 2354 |
|
Description: Anti-Rituximab Antibodies (recommended for ADA assay) antibody is produced from a hybridoma resulting from fusion of SP2/0 myeloma and B-lymphocytes obtained from a mouse immunized with Rituximab. |
Anti-Bevacizumab Antibody (AY9) (recommended for ADA assay) |
BEB-Y9 |
ACROBIOSYSTEMS |
100ug |
EUR 2354 |
|
Description: Anti-Bevacizumab Antibodies (recommended for ADA assay) antibody is produced from a hybridoma resulting from fusion of SP2/0 myeloma and B-lymphocytes obtained from a mouse immunized with Bevacizumab. |
Hyperactive pG-MNase CUT&RUN Assay Kit for Illumina |
HD102-01 |
Vazyme |
24 rxns |
EUR 316.8 |
Live/Dead Cell Viability Assay Kit (for Mammalian Cells) |
K502-100 |
Biovision |
each |
EUR 548.4 |